神经营养因子修饰胚胎脊髓神经干细胞移植后脊髓损伤大鼠的功能恢

[摘要] 目的 探究神经营养因子修饰胚胎脊髓神经干细胞移植后脊髓损伤大鼠的功能恢复与损伤局部基因表达。

方法 于2016年8月从孕18 d的30只大鼠胚胎脑部皮质中提取适量的脊髓神经干细胞，对其进行体外培养，然后采用巢蛋白免疫荧光染色方法进行鉴定，将鉴定后的胚胎脊髓神经干细胞植入成年的大鼠损伤脊髓，并将其分为3组，对照组10只、神经干细胞组10只、神经干细胞加胚胎细胞衍生的神经因子移植10只，对胚胎神经干细胞的存活进行观察分析，并对大鼠的损伤后的功能恢复情况以及局部基因表达进行比较分析。

结果 经过胚胎神经干细胞移植后，7、21 d大鼠的运动功功能评分分别为（26.37±4.62）分、（39.55±2.42）分，对照组7、21 d的运动功能评分分别为（25.62±2.523）分、（32.43±2.46）分，差异有统计学意义（P毕业论文网 　　[关键词] 神经营养因子；胚胎脊髓神经干细胞；脊髓损伤；功能恢复 　　[中图分类号] R446 [文献标识码] A [文章编号] 1674—0742（2017）09（b）—0001—03 　　[Abstract] Objective This paper tries to investigate the functional recovery and damage of local gene expression in rats with spinal cord injury after transplantation of neurotrophic factor—modified embryonic spinal cord neural stem cells. Methods The spinal cord neural stem cells were extracted from the brain cortex of 30 rat embryos for 18 days from August 2016， and then cultured in vitro by nestin immunofluorescence staining. The embryonic spinal cord neural stem cells were implanted into adult rats. The adult rats were divided into three groups： the control group （n=10）， the neural stem cells （n=10）， the neural stem cells （MSCs） and embryonic cells （n=10）. The survival of embryonic neural stem cells was observed and analyzed. And the functional recovery of the rats after injury and the local gene expression were analyzed. Results After embryonic neural stem cells transplantation， the movement function score of rats after 7 days and 21 days were （26.37±4.62）points and （39.55±2.42）points respectively， and the control group in the movement of 7 days and 21 days were （25.62±2.523）points and （32.43±2.46）points respectively， the difference was statistically significant （P。